The image consists of five panels labeled A through E. Panel A labeled H S P 104 H H 1 displays a kymograph with cell cycle arrest, condensation, anaphase onset, and tip growth labeled. Panel B presents corresponding fluorescence micrographs from the same time-lapse sequence, acquired every 10 seconds, highlighting ring-like localization, cytoplasmic extrusion, and apical nucleus. Panel C shows time-lapse micrographs of a hypha expressing H s p104 D W B-G F P and Histone H 1-R F P, illustrating stages like apical nucleus, tentative nucleolus, ring-like localization, chromatin condensation, anaphase nucleus, enrichment at nucleolar remnant, and remodeling of remnant. Panel D includes kymographs from a time-lapse sequence showing C e t1-R F P and H s p 104-G F P dynamics in a single hypha, with the upper panel showing merged channels and the lower panel showing H s p104-G F P alone. Panel E features a plot of relative fluorescence intensity over time for H s p 104-G F P and C e t 1-R F P. Both curves show a fluctuating pattern, with the curve for C e t 1 showing a declining trend, while the curve for H S P 104 shows an increasing trend. A dashed vertical line at the junction of the two curves is labeled M 1.
Hsp104 enters the nucleus at mitotic onset following heat shock recovery. (A) Kymograph from a time-lapse sequence showing nuclear (histone H1-RFP) and Hsp104-GFP dynamics in a single hypha recovering from a 45-min heat shock at 42°C. (B) Corresponding fluorescence micrographs from the same time-lapse sequence. Images were acquired every 10 s. (C) Time-lapse micrographs of a hypha expressing Hsp104DWB-GFP and histone H1-RFP. (D) Kymographs from a time-lapse sequence showing Cet1-RFP and Hsp104-GFP dynamics in a single hypha. Upper panel shows merged channels; lower panel shows Hsp104-GFP alone. (E) Plot of relative fluorescence intensity over time for Hsp104-GFP (green) and Cet1-RFP (magenta) within a nuclear ROI in the same hypha (n = 1 nucleus). M1 indicates the onset of mitosis.