Panels A and B show representative current traces for channel Activation and Inactivation, respectively. Each panel includes a voltage pulse protocol, black Control traces, and cyan M beta C D traces, with a scale bar indicating 1 n A and 5 m s. Panel C is a line graph showing the averaged G/G max relationships. The horizontal axis represents Voltage (millivolt) ranging from minus 70 to plus 10. Activation is plotted with triangle markers (ascending curves) and inactivation with diamond markers (descending curves). M beta C D (cyan) shows a visible leftward shift in activation compared to Control (black). Panels D and E are summary scatter plots for activation. Panel D shows Activation V 1/2 (millivolt), where M beta C D treatment significantly shifts the value (P = 0.01). Panel E shows Activation V s (millivolt) (P equals 0.16). Panels F and G are summary scatter plots for inactivation. Panel F shows Inactivation V 1/2 (millivolt) (P equals 0.18), and Panel G shows Inactivation V s (millivolt) (P equals 0.49). Individual data points are shown as dots, with horizontal lines representing the mean p m s.e.m. All values are approximate.
Effects of cholesterol extraction on the gating of endogenous sodium currents of naïve nociceptor DRG neurons. (A and B) Representative currents caused by sodium channel activation (A) and inactivation (B) in naïve nociceptor DRG neurons, before and after acute 2.5 mM mβCD treatment. (C) Averaged G-V relationships showing the activation (triangle maker) and inactivation (diamond shape) profiles of endogenous sodium channels before and after acute 2.5 mM mβCD treatment. (D and E) Summary data showing the V1/2 and the slope factor (Vs) for sodium channel activation. (F and G) Summary data showing the V1/2 and the slope factor (Vs) for sodium channel inactivation. Data shown are mean ± SEM, n = 5 patches, *P = 0.01 using two-sided paired t test.