Figure S6.
Control experiments examining HCN channel gating in naïve DRG nociceptors without WSC treatment, in comparison with conditions with WSC treatment. (A–C) Summary time course of the change in the V1/2 and slope factor (Vs) (A), the current amplitude (B), and τ1 of channel activation (C) after 0.5 mg/ml WSC application for naïve DRG neurons. Data shown are mean ± SEM, n = 7 patches. **P = 0.004 in panel A using two-sided paired t test. (D) Summary of the change in V1/2 (ΔV1/2) 5 min after with or without WSC treatment for naïve and SNI DRG neurons. Added 0.5 mM cAMP in the pipette solution was also included as a comparison, showing no difference from the condition without the added cAMP, suggesting the effect was mediated by WSC. Data shown are mean ± SEM, n = 5–7 patches, one-way ANOVA, **P = 1e−4 (naïve: WSC versus no treatment); **P = 0.007 (naïve versus SNI, with WSC). Refer to the image caption for details. Panel A is a time course graph depicting the time course of changes in the half-activation voltage (V 1/2) and slope factor (V s) over 15 minutes after applying W S C. The horizontal axis represents time in minutes, and the vertical axis represents V 1/2 in millivolts (left) and slope factor in millivolts (right). Panel B is a time course graph showing the normalized current amplitude over time, indicating a slight decrease, with the vertical axis representing normalized current amplitude from 0.0 to 1.0. Panel C is a time course showing the time constant of activation (tau 1) over time, with the vertical axis representing tau 1 activation in milliseconds. Panel D is a scatter plot summarizing the change in V 1/2 (Delta V 1/2) five minutes after treatment across four conditions: Naive with W S C, Naive no treatment, Naive c A M P with W S C, and S N I with W S C. The y-axis represents the change in V 1/2 in millivolts, ranging from 0 to minus 14. Individual data points are shown as dots, with horizontal bars indicating mean p m s.e.m. Significant differences compared to the Naive with W S C group are marked with double asterisks. All values are approximate.

Control experiments examining HCN channel gating in naïve DRG nociceptors without WSC treatment, in comparison with conditions with WSC treatment. (A–C) Summary time course of the change in the V1/2 and slope factor (Vs) (A), the current amplitude (B), and τ1 of channel activation (C) after 0.5 mg/ml WSC application for naïve DRG neurons. Data shown are mean ± SEM, n = 7 patches. **P = 0.004 in panel A using two-sided paired t test. (D) Summary of the change in V1/2 (ΔV1/2) 5 min after with or without WSC treatment for naïve and SNI DRG neurons. Added 0.5 mM cAMP in the pipette solution was also included as a comparison, showing no difference from the condition without the added cAMP, suggesting the effect was mediated by WSC. Data shown are mean ± SEM, n = 5–7 patches, one-way ANOVA, **P = 1e−4 (naïve: WSC versus no treatment); **P = 0.007 (naïve versus SNI, with WSC).

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