P-α-syn pathology varies spatially and temporally after injection of α-syn mpffs and AD-tau–enriched extracts under copathology conditions. (A–D) Representative images for mAb 81A p-α-syn pathology in tissue sections from rostral and caudal hippocampus (A), entorhinal cortex (B), retrosplenial cortex (C), and auditory cortex (D) of WT mice at 3, 6, or 9 mpi of either α-syn mpffs alone or combined with AD-tau–enriched extracts. (E–L) Quantification of p-α-syn seen in A–D in the ipsilateral (E) and contralateral (F) hippocampus, ipsilateral (G) and contralateral (H) entorhinal cortex, ipsilateral (I) and contralateral (J) retrosplenial cortex, and ipsilateral (K) and contralateral (L) auditory cortex. (M) Semiquantitative heat mapping of p-α-syn pathology (see Materials and methods for details) in WT mice injected with either α-syn mpffs alone or α-syn mpffs combined with human AD-tau–enriched extracts. A two-tailed t test was performed to calculate the difference between groups; *, P < 0.05. Data are presented as mean ± SEM (n = 5 mice per group). Scale bar, 100 µm. All experimental data were verified in at least two independent experiments.