Reconstitution of patient fibroblasts with WT OSMR restores OSMR surface expression and STAT3 phosphorylation. Lentiviral transduction of patient dermal fibroblasts with WT and variant OSMR and GFP as transduction control. (A) The transduced fibroblasts were stimulated with 5 ng/ml OSM for 30 min, and whole-cell lysates were harvested for immunoblotting for the visualization of pSTAT3, STAT3, OSMR, GFP, and GAPDH, which was used as loading control. (B) WB band intensities of pSTAT3/STAT3 from A were determined by ImageJ. (C and D) (C) Surface expression of OSMR from the lentiviral transduced fibroblasts was analyzed by FC, and (D) MFI values were plotted. The data shown are representative of three independent experiments performed in duplicates.  HC, healthy control; MFI, mean of fluorescence intensities. Source data are available for this figure: SourceData F6.