Gating strategies for B cell subsets and CSR analyses. (A) Gating strategy for B cell subset analyses of PBMCs. (Data shown in row 1, columns 4 and 5 are identical to data shown in Fig. 5A, rows 1 and 2, column 1.) (B) Gating strategy for CSR analyses from full blood. aMBC_PC, aMBC/plasma cell; (a)swMBC, (atypical) switched memory B cell; auswMBC, atypical unswitched memory B cell; DN, double-negative B cell subset; dbneg, double-negative for markers in respective bivariate plot; MBC_only, conventional memory B cell; SWmem, switched memory; T1, T2, T3, transitional 1, 2, 3 subsets.