Gating strategies and flow analysis of T cell subsets of Th17, Th9, Th2, and pTFH cell frequencies. (A) Gating strategy for Th17 cells, classified as CD4⁺CD161⁺ CCR4⁺CCR6⁺. The first row shows representative flow plots for five HCs, and the second row shows flow plots for P1. (B) Th17 frequencies of CD4⁺. (C) Gating strategy for Th9 cells, classified as CD4⁺ CXCR3 (indicated as CXCR5 in dot plot)⁺ CCR4⁻ CCR6⁺. The first row shows representative flow plots for three HCs, and the second row shows flow plots for P1. Duplicate of data shown in A, rows 1 and 2. (D) Th9 frequencies of CD4⁺. (E) Gating strategy for Th2 cells, classified as CD4⁺ CD124 (IL-4Ra, indicated as CCR4 in dot plot). The first row shows representative flow plots for three HCs, and the second row shows flow plots for P1. (F) Th2 frequencies of CD4⁺. (G) Gating strategy for pTFH cells classified as CD4 (APC)⁺ CXCR5 (Per-CP)⁺ ICOS (BV421)⁺ PD-1⁺ (PE) in dot plot. (H) pTFH cell frequencies of CD4⁺. HC, healthy control.