Single-cell RNA sequencing identifies GPR183-expressing monocytes in human airway disease. (A) UMAP showing the indicated lymphoid and myeloid cell clusters (14,437 cells) in BAL fluid after integrating single-cell RNA-sequencing datasets from six bronchiectasis patients (this study) and 10 healthy donors (Mould et al., 2021) as described in the Materials and methods. MΦ, macrophage; mono, monocyte. (B) Violin plots showing the expression of the indicated genes in the myeloid clusters from panel A. (C) Alveolar macrophage and CD14⁺CD16⁻ lung monocyte signature (Li et al., 2022b) scores in the myeloid clusters from panel A. (D) Signature scores of human lung macrophages either derived from CD116⁺ fetal precursors or from CD34⁺ HSPCs (Evren et al., 2022) in the myeloid clusters from panel A. (E) Relative contribution of healthy (n = 10) versus bronchiectasis (n = 6) donors to each cluster in the BAL fluid. Data are represented as mean ± SEM. **P < 0.01; ***P < 0.001; ****P < 0.0001 by unpaired Student’s t test. (F) Lineage inference analysis with slingshot of the myeloid clusters from panel A. Data in panels A–F are from one single-cell RNA-sequencing experiment with n = 6 human bronchiectasis donors that was integrated with the dataset of n = 10 healthy human donors by Mould et al. (2021).