Expression of Ccl2 and Csf1 in SPAM deleter mice after alveolar macrophage depletion. (A) Dot plot showing expression of marker genes to define endothelial cells, epithelial cells, and fibroblasts in the lung of IM-DTR mice as in Fig. 8, F–G. Data are from one single-cell RNA-sequencing experiment with n = 3–4 mice per time point. (B and C) Time course of Ccl2 (A) and Csf1 (B) expression in the lung clusters of SPAM deleter mice from Fig. 3 E. Data are from one single-cell RNA-sequencing experiment with n = 4 mice per time point. IM, interstitial macrophage.