Cell type–specific requirement of GPR183 expression for monocyte-derived alveolar macrophages. (A) Overview of mixed Gpr183^+/+/Rag1^−/− and Gpr183^−/−/Rag1^−/− bone marrow chimeras. In mixed Gpr183^−/−/Rag1^−/− chimeras, lymphocytes (T and B cells) lack Gpr183 expression, whereas in control Gpr183^+/+/Rag1^−/− chimeras Gpr183 expression is preserved in T and B lymphocytes. (B) Chimerism of donor-derived monocytes and alveolar macrophages in mixed Gpr183^+/+/Rag1^−/− and Gpr183^−/−/Rag1^−/− chimeras 10 wk after bone marrow transfer. Gating strategy is shown in Fig. S1 C. Data are represented as mean ± SEM. ns, not significant; *P < 0.05 by unpaired Student’s t test. Data are pooled from two independent bone marrow chimera experiments with a total of n = 4 mice per genotype. (C) Overview of mixed Gpr183^+/+/Ccr2^RFP/RFP and Gpr183^−/−/Ccr2^RFP/RFP bone marrow chimeras. Monocytes derived from Ccr2^RFP/RFP bone marrow have defective bone marrow egress and therefore lung monocytes in mixed Gpr183^−/−/Ccr2^RFP/RFP chimeras lack Gpr183 expression. (D) Chimerism of donor-derived alveolar macrophages in mixed Gpr183^+/+/Ccr2^RFP/RFP and Gpr183^−/−/Ccr2^RFP/RFP chimeras 9–10 wk after bone marrow transfer. Gating strategy is shown in Fig. S1 C. Data are represented as mean ± SEM. ns, not significant by unpaired Student’s t test. Data are pooled from two independent bone marrow chimera experiments with a total of n = 9 mice per genotype. Panels A and C were adapted from Servier Medical Art.