Gating strategy for monocytes and macrophages in tissues other than lung. (A–E) Gating strategies to identify the indicated monocyte and macrophage populations in Fig. 4 E in (A) brain, (B) blood, (C) spleen, (D) peritoneal cavity, and (E) liver. Data are representative of one (peritoneal cavity), three (brain), four (liver), or five (spleen) independent bone marrow chimera experiments with a total of n = 6–32 mice per tissue.