Figure 4.
GPR183 promotes the development of monocyte-derived macrophages in the lung. (A and B) Chimerism of monocytes, alveolar macrophages, interstitial macrophages, and other lung immune cells in competitive Gpr183+/+/Gpr183−/− chimeras 9–13 wk after bone marrow transfer. Injected bone marrow input is shown in the upper left flow cytometry dot plot of panel A. Gating strategy is shown in Fig. S1 C. The ratio of the indicated Gpr183+/+/Gpr183−/− cells in panel B was normalized to the bone marrow input of each independent chimera experiment. AMs, alveolar macrophages; IMs, interstitial macrophages; PMNs, polymorphonuclear neutrophils. Data are represented as mean ± SEM. ****P < 0.0001 by one-way ANOVA with Tukey’s multiple comparison post hoc test. Data are pooled from three independent bone marrow chimera experiments with a total of n = 17–19 mice. (C) Chimerism of Ly6Chi monocytes and alveolar macrophages in the lung of competitive Gpr183+/+/Gpr183−/− chimeras 19–23 wk after bone marrow transfer. Gating strategy is shown in Fig. S1 C. The ratio of the indicated Gpr183+/+/Gpr183−/− cells was normalized to the bone marrow input of each independent chimera experiment. Data are represented as mean ± SEM. ns, not significant; ****P < 0.0001 by one-way ANOVA with Tukey’s multiple comparison post hoc test. Data are pooled from two independent bone marrow chimera experiments with a total of n = 14–15 mice. (D) Number of CD45.2+ lung monocytes and macrophages in single-transfer Gpr183+/+ (CD45.2+) → B6 (CD45.1+) and Gpr183−/− (CD45.2+) → B6 (CD45.1+) chimeras 7–8 wk after bone marrow transfer. Gating strategy is shown in Fig. S1 C. Data are represented as mean ± SEM. ns, not significant by unpaired Student’s t test. Data are pooled from two independent experiments from a single bone marrow chimera experiment with a total of n = 4–5 mice per genotype. (E) Chimerism of Ly6Chi monocytes and macrophages in the indicated tissues of competitive Gpr183+/+/Gpr183−/− chimeras 9–29 wk after bone marrow transfer. Macs, macrophages; monos, monocytes. Gating strategy is shown in Fig. S3. Data are represented as mean ± SEM. ns, not significant; ***P < 0.001; ****P < 0.0001 by one-way ANOVA with Tukey’s Multiple Comparison post hoc test (spleen, peritoneal cavity, and liver) or by unpaired Welch’s t test (brain). Data are pooled from one (peritoneal cavity), three (brain), four (liver), or five (spleen) independent bone marrow chimera experiments with a total of n = 6–32 mice per tissue.

GPR183 promotes the development of monocyte-derived macrophages in the lung. (A and B) Chimerism of monocytes, alveolar macrophages, interstitial macrophages, and other lung immune cells in competitive Gpr183+/+/Gpr183−/− chimeras 9–13 wk after bone marrow transfer. Injected bone marrow input is shown in the upper left flow cytometry dot plot of panel A. Gating strategy is shown in Fig. S1 C. The ratio of the indicated Gpr183+/+/Gpr183−/− cells in panel B was normalized to the bone marrow input of each independent chimera experiment. AMs, alveolar macrophages; IMs, interstitial macrophages; PMNs, polymorphonuclear neutrophils. Data are represented as mean ± SEM. ****P < 0.0001 by one-way ANOVA with Tukey’s multiple comparison post hoc test. Data are pooled from three independent bone marrow chimera experiments with a total of n = 17–19 mice. (C) Chimerism of Ly6Chi monocytes and alveolar macrophages in the lung of competitive Gpr183+/+/Gpr183−/− chimeras 19–23 wk after bone marrow transfer. Gating strategy is shown in Fig. S1 C. The ratio of the indicated Gpr183+/+/Gpr183−/− cells was normalized to the bone marrow input of each independent chimera experiment. Data are represented as mean ± SEM. ns, not significant; ****P < 0.0001 by one-way ANOVA with Tukey’s multiple comparison post hoc test. Data are pooled from two independent bone marrow chimera experiments with a total of n = 14–15 mice. (D) Number of CD45.2+ lung monocytes and macrophages in single-transfer Gpr183+/+ (CD45.2+) → B6 (CD45.1+) and Gpr183−/− (CD45.2+) → B6 (CD45.1+) chimeras 7–8 wk after bone marrow transfer. Gating strategy is shown in Fig. S1 C. Data are represented as mean ± SEM. ns, not significant by unpaired Student’s t test. Data are pooled from two independent experiments from a single bone marrow chimera experiment with a total of n = 4–5 mice per genotype. (E) Chimerism of Ly6Chi monocytes and macrophages in the indicated tissues of competitive Gpr183+/+/Gpr183−/− chimeras 9–29 wk after bone marrow transfer. Macs, macrophages; monos, monocytes. Gating strategy is shown in Fig. S3. Data are represented as mean ± SEM. ns, not significant; ***P < 0.001; ****P < 0.0001 by one-way ANOVA with Tukey’s Multiple Comparison post hoc test (spleen, peritoneal cavity, and liver) or by unpaired Welch’s t test (brain). Data are pooled from one (peritoneal cavity), three (brain), four (liver), or five (spleen) independent bone marrow chimera experiments with a total of n = 6–32 mice per tissue.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal