Myenteric inflammation in mice treated with the HIF1α inhibitor PX-478 or with neuron-specific depletion of Myd88, Hif1a, or Vhl. (A) Complementary to Fig. 6 K: FC plots showing colonic Mo-MM subsets and their percentages in 1×DSS- or water-treated WT mice injected daily with PX-478 or vehicle. (B) Percentage of total viable cells (left) and absolute cell counts (right) of colonic M-PMNs in mice described in A, quantified by FC (DSS vehicle and PX-478, n = 6 per group). Baseline shows mean value for PX-478–injected mice on water (n = 1). (C) FC plots showing colonic Mo-MM subsets and their percentages in tamoxifen- and 1×DSS- (3%) or water-treated SLICK-H^ER-CreHif1a^fl/fl (Cre⁺) or Hif1a^fl/fl (Cre^–) littermates. (D) Percentage of total viable cells (left) and absolute cell counts (right) of colonic Mo-MMs in mice described in C, quantified by FC (DSS Cre^– and Cre⁺, n = 17 per group). Baseline shows the mean value for tamoxifen- and water-treated Hif1a^fl/fl littermates (Water Cre^–, n = 13). P values above each column show statistical differences against baseline. (E) Percentage of total viable cells (left) and absolute cell counts (right) of colonic M-PMNs in mice described in C (DSS Cre^– and Cre⁺, n = 17 per group), quantified by FC (baseline Water Cre^–, n = 13). P values above each column show statistical differences against baseline. (F) FC plots of colonic Mo-MM subsets and their percentages in tamoxifen- and 1×DSS- (3%) or water-treated SLICK-H^ER-CreMyd88^fl/fl (Cre⁺) or Myd88^fl/fl (Cre^–) littermates. (G) Percentage of total viable cells (left) and absolute cell counts (right) of colonic Mo-MMs in mice described in F, quantified by FC (DSS Cre^– and Cre⁺, n = 15 per group). Baseline shows the mean value for tamoxifen- and water-treated Myd88^fl/fl littermates (Water Cre^–, n = 12). P values above each column show statistical differences against baseline. (H) Percentage of total viable cells (left) and absolute cell counts (right) of colonic M-PMNs in mice described in F (DSS Cre^– and Cre⁺, n = 15 per group), quantified by FC (baseline water Cre^–, n = 12). P values above each column show statistical differences against baseline. (I) Complementary to Fig. 6 M: FC plots of colonic Mo-MM subsets and their percentages in tamoxifen- and 3×DSS- or water-treated SLICK-H^ER-CreVhl^fl/fl (Cre⁺) or Vhl^fl/fl (Cre^–) littermates. (J) Percentage of total viable cells (top) and absolute cell counts (bottom) of colonic M-PMNs in mice described in I, quantified by FC (DSS Cre^– and Cre⁺, n = 18–19 per group). Baseline shows the mean value for tamoxifen- and water-treated Vhl^fl/fl littermates (baseline water Cre^–, n = 5–9). All graphs show the mean ± SEM, n—mouse. Data are representative of at least two independent experiments with similar results. Data are pooled from 2 to 4 smaller experiments (B, D, E, G, H, and J). Statistical analyses: unpaired Student’s t test (B, D, E, G, H, and J), *P ≤ 0.05, **P ≤ 0.01, and ****P ≤ 0.0001.