Tamoxifen-inducible Cre expression in SLICK-H ^ER-Cre mice selectively targets neurons in the colonic myenteric plexus. (A) Confocal images of colonic myenteric plexus from untreated (control) R26-STOP^fl/fltdTomato (Cre^–, left) and SLICK-H^ER-CreR26-STOP^fl/fltdTomato (Cre⁺, right) mice, stained with βIII-tubulin antibody (nerve fibers), endogenous SLICK-H-YFP (YFP), and DAPI (nuclei). Scale bars, 100 μm. (B) FC plots showing percentage of YFP⁺ and tdTomato⁺ cells among CD45^– cells in colonic muscularis of tamoxifen-treated control Cre^– (left) and Cre⁺ (right) mice described in A. (C) Confocal images of colonic myenteric plexus from tamoxifen-treated control Cre^– (left) and Cre⁺ (right) mice described in A, stained with βIII-tubulin (nerve fibers), tdTomato (Cre-targeted cells), and PGP9.5 (whole neurons) antibodies. Scale bars, 100 μm. (D) Confocal images of colonic myenteric plexus from tamoxifen-treated Cre⁺ mice described in A, stained with s100β (enteric glia), tdTomato (Cre-targeted cells), and PGP9.5 (whole neurons) antibodies. Scale bars, 100 μm. (E) FC plots showing percentage of CD90⁺CD24⁺ cells among total CD45^– SLICK-H:tdTomato⁺ cells (left) or SLICK-H:tdTomato⁺ cells among total CD45^–CD90⁺CD24⁺ cells (right) in colonic muscularis of tamoxifen-treated Cre⁺ mice described in A.