No differences in the percentage of T_regsafter MOG immunization. (A–C) Example of the gating for FoxP3⁺ T_regs in the dLN at 8 dpi with MOG_35–55/CFA and PTx. The percentages of T_regs in the (B) spleen and dLNs at 3 dpi and (C) 8 dpi. (D) Percentages of T_regs in the CNS, dLN, and spleen during the peak of EAE. T_regs were pregated on CD45⁺, TCRβ⁺ or CD90⁺, and CD4⁺ cells; dead cells and duplets were excluded. Data in graphs are shown as the mean ± SEM and analyzed using two-way ANOVA with Šídák’s multiple comparisons test. dpi = days after immunization.  Each dot represents one mouse. Data are from three independent experiments.