Figure S1.
Neutrophils and monocytes at the peak of EAE. (A and B) Percentage of RFP-expressing cells in different populations in the RFP CX3CR1-Cre and (B) RFP CD11c-Cre in the dLNs 5 days after MOG immunization. (C) Gating for Ly6G+ neutrophils and Ly6C+ monocytes in the CNS and dLN, gated on CD11b+/live/single cells. (D and E) Cell number and percentages of neutrophils and (E) monocytes in the CNS, dLN, and spleen. (F–H) α-Diversity (Shannon index) of fecal microbiota from naïve control and NIKΔCX3CR1 mice housed under identical SPF conditions. (G) β-Diversity analysis visualized by weighted UniFrac PCoA. (H) Relative abundance of dominant bacterial taxa at the genus level. Data in A, B, D, and E are shown as the mean ± SEM and analyzed using two-way ANOVA with Šídák’s multiple comparisons test. **P < 0.01, ****P < 0.0001. Each dot represents one mouse. Data are from three independent experiments. PCoA, principal coordinates analysis. Refer to the image caption for details.

Neutrophils and monocytes at the peak of EAE. (A and B) Percentage of RFP-expressing cells in different populations in the RFP CX3CR1-Cre and (B) RFP CD11c-Cre in the dLNs 5 days after MOG immunization. (C) Gating for Ly6G+ neutrophils and Ly6C+ monocytes in the CNS and dLN, gated on CD11b+/live/single cells. (D and E) Cell number and percentages of neutrophils and (E) monocytes in the CNS, dLN, and spleen. (F–H) α-Diversity (Shannon index) of fecal microbiota from naïve control and NIKΔCX3CR1 mice housed under identical SPF conditions. (G) β-Diversity analysis visualized by weighted UniFrac PCoA. (H) Relative abundance of dominant bacterial taxa at the genus level. Data in A, B, D, and E are shown as the mean ± SEM and analyzed using two-way ANOVA with Šídák’s multiple comparisons test. **P < 0.01, ****P < 0.0001. Each dot represents one mouse. Data are from three independent experiments. PCoA, principal coordinates analysis.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal