GFPpkp4-E-cadherin clusters in α-catenin–deficient cells display characteristics of lateral AJs. (a) Confocal fluorescence microscopy (maximum-intensity projections of all x–y slices) of αCat-KO cells expressing αCatCH-Δ259 triple stained for mCherry, pkp4, and vinculin. Bar, 20 μm. Detailed subcellular distribution of pkp4 (red) and vinculin (green) within the regions marked by white dashed boxes are shown below. Bar, 10 μm. (b) Maximum-intensity projections of all x–y optical slices of the same cells double-stained for pkp4 (red) and erbin or PLEKHA5 (green). Only merged images are shown at low magnifications. Bar, 20 μm. Enlarged areas (indicated by white dashed boxes) are shown at right for both channels. Bar, 10 μm. αCat, α-catenin; mCH, mCherry tag.