Phenotype of EcGFP-A431 cells deficient for pkp4 and p120. (a) Maximum-intensity projections of all x–y optical slices of the p120-KO and pkp4-KO EcGFP-A431 cells stained for EcGFP (GFP, green) and pkp4 or p120 (both red), respectively. Only merged images are shown for low magnifications. Bar, 20 μm. The zoomed areas (marked by dashed boxes) are shown at right in both colors. Bar, 10 μm. (b) The scatterplots of red-green relative pixel intensities of the images shown in a. The Pearson’s r values are in the upper right corners. Note that the cells show nearly perfect positive relationship between the fluorophores. (c) Projections of all x–y slices of the same knockout cells stained for EcGFP (green) and vinculin (red). Only merged images are shown at low magnification. Bar, 20 μm. Enlarged regions (dashed boxes) are presented below in both channels. Bar, 15 μm. Its dashed box area is further magnified in the bottom row. Bar, 8 μm. Arrows indicate vinculin-positive lateral AJs observed in pkp4-KO cells.