Figure 6.
IL-1β–responsive BNST neurons are necessary for stress-induced inflammatory responses. (A) Schematic of ablating IL-1β–responsive BNST neurons bilaterally in TRAP2 mice and then exposure to restraint stress. (B) Serum IL-6 levels in TRAP2 mice: naive group without restraint stress, after 4-h restraint stress in the control dtA (−) and IL-1β–responsive BNST neuron-ablated dtA (+) mice. Data are represented as individual mouse data points from two independent experiments. One-way ANOVA with Tukey’s multiple comparison test. (C) ΔHR under restraint stress in IL-1β–responsive neuron-ablated group (red) and control group (black) (control, n = 5 mice; dtA, n = 5 mice, mixed-effects analysis with Šidák correction). (D) AUC of ΔHR under restraint stress conditions in the IL-1β–responsive BNST neuron-ablated group and control group. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (E) Serum corticosterone levels after 4-h restraint stress. Data are represented as individual mouse data points pooled from two independent experiments. One-way ANOVA with Tukey’s multiple comparison test. (F) Schematic of ablating CRH+ BNST neurons bilaterally in crh-cre mice and then exposure to retrain stress. (G) Serum IL-6 levels at 4 h after restraint stress in crh-cre mice: naive group without restraint stress, control dtA (−), and CRH+ BNST neuron-ablated dtA (+) mice. Data are represented as individual mouse data points pooled from two independent experiments. One-way ANOVA with Tukey’s multiple comparison test. (H) ΔHR under restraint stress in control (black) and CRH+ neuron-ablated group (red) (control, n = 6 mice; dtA, n = 5 mice, P = 0.078, mixed-effects analysis with Šidák correction). (I) AUC of ΔHR under restraint stress. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (J) Serum corticosterone levels at 4-h postrestraint stress in control, CRH+ BNST neuron-ablated group, and control group without restraint stress. Data are represented as individual mouse data points pooled from two independent experiments. One-way ANOVA with Tukey’s multiple comparison test. *P < 0.05, **P < 0.01, and ***P < 0.001.

IL-1β–responsive BNST neurons are necessary for stress-induced inflammatory responses. (A) Schematic of ablating IL-1β–responsive BNST neurons bilaterally in TRAP2 mice and then exposure to restraint stress. (B) Serum IL-6 levels in TRAP2 mice: naive group without restraint stress, after 4-h restraint stress in the control dtA (−) and IL-1β–responsive BNST neuron-ablated dtA (+) mice. Data are represented as individual mouse data points from two independent experiments. One-way ANOVA with Tukey’s multiple comparison test. (C) ΔHR under restraint stress in IL-1β–responsive neuron-ablated group (red) and control group (black) (control, n = 5 mice; dtA, n = 5 mice, mixed-effects analysis with Šidák correction). (D) AUC of ΔHR under restraint stress conditions in the IL-1β–responsive BNST neuron-ablated group and control group. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (E) Serum corticosterone levels after 4-h restraint stress. Data are represented as individual mouse data points pooled from two independent experiments. One-way ANOVA with Tukey’s multiple comparison test. (F) Schematic of ablating CRH+ BNST neurons bilaterally in crh-cre mice and then exposure to retrain stress. (G) Serum IL-6 levels at 4 h after restraint stress in crh-cre mice: naive group without restraint stress, control dtA (−), and CRH+ BNST neuron-ablated dtA (+) mice. Data are represented as individual mouse data points pooled from two independent experiments. One-way ANOVA with Tukey’s multiple comparison test. (H) ΔHR under restraint stress in control (black) and CRH+ neuron-ablated group (red) (control, n = 6 mice; dtA, n = 5 mice, P = 0.078, mixed-effects analysis with Šidák correction). (I) AUC of ΔHR under restraint stress. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (J) Serum corticosterone levels at 4-h postrestraint stress in control, CRH+ BNST neuron-ablated group, and control group without restraint stress. Data are represented as individual mouse data points pooled from two independent experiments. One-way ANOVA with Tukey’s multiple comparison test. *P < 0.05, **P < 0.01, and ***P < 0.001.

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