Figure 5.
PVN signaling is necessary for mediating the effects of IL-1β–responsive BNST neurons. (A) Schematic for chemogenetic reactivation of IL-1β–responsive BNST neurons with ablation of IL-1β–responsive PVN neurons in TRAP2 mice. (B) Representative images showing IL-1β–responsive PVN neurons (c-Fos expression in green) after CNO administration in control dtA (−) and IL-1β–responsive neuron-ablated dtA (+) mice. Scale bar, 100 μm. (C) Serum IL-6 levels at 2 h after reactivation in naive mice, the control group, and with ablation of IL-1β–responsive PVN neurons. Data are represented as individual mouse data points pooled from three independent experiments. One-way ANOVA, Tukey’s multiple comparison test. (D) ΔHR for 60 min after reactivation of IL-1β–responsive BNST neurons with and without ablation of IL-1β–responsive PVN neurons: control (black) or dtA (red) (control, n = 5 mice; dtA, n = 5 mice, mixed-effects analysis with Šidák correction). (E) AUC of ΔHR after reactivation. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (F) Serum corticosterone levels at 2 h after reactivation in naive mice, the control group without ablation, and with ablation of IL-1β–responsive PVN neurons. Data are represented as individual mouse data points from three independent experiments. One-way ANOVA. (G) Schematic for chemogenetic activation of CRH+ BNST neurons with ablation of CRH+ PVN neurons in crh-cre mice. (H) Representative images showing the expression of c-Fos (green) in the PVN after CNO administration in control dtA (−) and CRH+ PVN neuron-ablated dtA (+) mice. Scale bar, 100 μm. (I) Serum IL-6 levels at 2 h after reactivation in crh-cre naive mice, control mice without ablation, and mice with ablation of CRH+ PVN neurons. Data are represented as individual mouse data points pooled from two independent experiments. One-way ANOVA, Tukey’s multiple comparison test. (J) ΔHR for 60 min after chemogenetic activation of CRH+ BNST neurons with ablation of CRH+ PVN neurons: control (black) or dtA (red) (control, n = 5 mice; dtA, n = 7 mice, mixed-effects analysis with Šidák correction). (K) AUC of ΔHR after reactivation in crh-cre mice. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (L) Serum corticosterone levels at 2 h after reactivation in crh-cre naive mice, control group without ablation, and with ablation of IL-1β–responsive PVN neurons. Data are represented as individual mouse data points pooled from two independent experiments. One-way ANOVA. *P < 0.05 and **P < 0.01.

PVN signaling is necessary for mediating the effects of IL-1β–responsive BNST neurons. (A) Schematic for chemogenetic reactivation of IL-1β–responsive BNST neurons with ablation of IL-1β–responsive PVN neurons in TRAP2 mice. (B) Representative images showing IL-1β–responsive PVN neurons (c-Fos expression in green) after CNO administration in control dtA (−) and IL-1β–responsive neuron-ablated dtA (+) mice. Scale bar, 100 μm. (C) Serum IL-6 levels at 2 h after reactivation in naive mice, the control group, and with ablation of IL-1β–responsive PVN neurons. Data are represented as individual mouse data points pooled from three independent experiments. One-way ANOVA, Tukey’s multiple comparison test. (D) ΔHR for 60 min after reactivation of IL-1β–responsive BNST neurons with and without ablation of IL-1β–responsive PVN neurons: control (black) or dtA (red) (control, n = 5 mice; dtA, n = 5 mice, mixed-effects analysis with Šidák correction). (E) AUC of ΔHR after reactivation. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (F) Serum corticosterone levels at 2 h after reactivation in naive mice, the control group without ablation, and with ablation of IL-1β–responsive PVN neurons. Data are represented as individual mouse data points from three independent experiments. One-way ANOVA. (G) Schematic for chemogenetic activation of CRH+ BNST neurons with ablation of CRH+ PVN neurons in crh-cre mice. (H) Representative images showing the expression of c-Fos (green) in the PVN after CNO administration in control dtA (−) and CRH+ PVN neuron-ablated dtA (+) mice. Scale bar, 100 μm. (I) Serum IL-6 levels at 2 h after reactivation in crh-cre naive mice, control mice without ablation, and mice with ablation of CRH+ PVN neurons. Data are represented as individual mouse data points pooled from two independent experiments. One-way ANOVA, Tukey’s multiple comparison test. (J) ΔHR for 60 min after chemogenetic activation of CRH+ BNST neurons with ablation of CRH+ PVN neurons: control (black) or dtA (red) (control, n = 5 mice; dtA, n = 7 mice, mixed-effects analysis with Šidák correction). (K) AUC of ΔHR after reactivation in crh-cre mice. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (L) Serum corticosterone levels at 2 h after reactivation in crh-cre naive mice, control group without ablation, and with ablation of IL-1β–responsive PVN neurons. Data are represented as individual mouse data points pooled from two independent experiments. One-way ANOVA. *P < 0.05 and **P < 0.01.

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