Figure 3.
Adrenergic signaling mediates CRH+ BNST neuron effects. (A) Schematic for chemogenetic activation of IL-1β–responsive BNST neurons in TRAP2 mice. (B) Serum IL-6 levels at 2 h after reactivation with AR blockers (propranolol, SR59230A; both administered at 5 mg/kg i.p.). Data are represented as individual mouse data points pooled from two independent experiments. One-way ANOVA, Tukey’s multiple comparison test. (C) ΔHR for 60 min after reactivation of IL-1β–responsive BNST neurons with DMSO (black) as a control, propranolol (red), or SR59230A (blue) (DMSO, n = 5 mice; propranolol, n = 5 mice; SR59230A, n = 5 mice, mixed-effects analysis with Šidák correction). (D) AUC of ΔHR after reactivation of IL1-responsive BNST neurons with AR blockers. Data are represented as individual mouse data points pooled from two independent experiments. One-way ANOVA, Tukey’s multiple comparison test. (E) Serum corticosterone levels at 2 h after reactivation of IL1-responsive BNST neurons with AR blockers. Data are represented as individual mouse data points pooled from two independent experiments. One-way ANOVA, Tukey’s multiple comparison test. (F) Schematic for pharmacogenetic activation of BNST neurons in crh-cre mice. (G) Serum IL-6 levels at 2 h after activation of CRH-positive BNST neurons with AR blockers in crh-cre mice. (H) ΔHR for 60 min after activation of CRH+ BNST neurons with DMSO (black) as a control, propranolol (red), or SR59230A (blue) (DMSO, n = 4 mice; propranolol, n = 5 mice; SR59230A, n = 5 mice, mixed-effects analysis with Šidák correction). (I) AUC of ΔHR after activation with AR blockers in crh-cre mice. Data are represented as individual mouse data points pooled from two independent experiments. One-way ANOVA, Tukey’s multiple comparison test. (J) Serum corticosterone levels at 2 h after activation of CRH-positive BNST neurons with AR blockers in crh-cre mice. Data are represented as individual mouse data points pooled from two independent experiments. One-way ANOVA, Tukey’s multiple comparison test. *P < 0.05; **P < 0.01.

Adrenergic signaling mediates CRH+ BNST neuron effects. (A) Schematic for chemogenetic activation of IL-1β–responsive BNST neurons in TRAP2 mice. (B) Serum IL-6 levels at 2 h after reactivation with AR blockers (propranolol, SR59230A; both administered at 5 mg/kg i.p.). Data are represented as individual mouse data points pooled from two independent experiments. One-way ANOVA, Tukey’s multiple comparison test. (C) ΔHR for 60 min after reactivation of IL-1β–responsive BNST neurons with DMSO (black) as a control, propranolol (red), or SR59230A (blue) (DMSO, n = 5 mice; propranolol, n = 5 mice; SR59230A, n = 5 mice, mixed-effects analysis with Šidák correction). (D) AUC of ΔHR after reactivation of IL1-responsive BNST neurons with AR blockers. Data are represented as individual mouse data points pooled from two independent experiments. One-way ANOVA, Tukey’s multiple comparison test. (E) Serum corticosterone levels at 2 h after reactivation of IL1-responsive BNST neurons with AR blockers. Data are represented as individual mouse data points pooled from two independent experiments. One-way ANOVA, Tukey’s multiple comparison test. (F) Schematic for pharmacogenetic activation of BNST neurons in crh-cre mice. (G) Serum IL-6 levels at 2 h after activation of CRH-positive BNST neurons with AR blockers in crh-cre mice. (H) ΔHR for 60 min after activation of CRH+ BNST neurons with DMSO (black) as a control, propranolol (red), or SR59230A (blue) (DMSO, n = 4 mice; propranolol, n = 5 mice; SR59230A, n = 5 mice, mixed-effects analysis with Šidák correction). (I) AUC of ΔHR after activation with AR blockers in crh-cre mice. Data are represented as individual mouse data points pooled from two independent experiments. One-way ANOVA, Tukey’s multiple comparison test. (J) Serum corticosterone levels at 2 h after activation of CRH-positive BNST neurons with AR blockers in crh-cre mice. Data are represented as individual mouse data points pooled from two independent experiments. One-way ANOVA, Tukey’s multiple comparison test. *P < 0.05; **P < 0.01.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal