snRNA-seq of BNST cells. (A) A UMAP plot of BNST cells with 17 cell clusters identified by snRNA-seq. (B) UMAP plots showing expression of indicated genes. The clusters showing high expression in Fig. 2 B are marked in red. (C) Representative images showing CRH expression in IL-1β–responsive BNST neurons: CRH (green), IL-1β–responsive BNST neurons (tdTomato, red), and DAPI (blue). Right panel shows the percentage of tdTomato⁺CRH⁺ double-positive cells in tdTomato⁺ cells in the BNST. Scale bar, 10 μm.