IL-1β–responsive BNST neurons are required for IL-1β–induced changes in heart rate, but not in serum IL-6 levels and core body temperature. (A) Schematic for ablation of IL-1β–responsive BNST neurons in TRAP2 mice. (B) AUC of ΔHR after PBS or IL-1β injection in each group (naïve, n = 5 mice; dtA[−], n = 5 mice; dtA[+], n = 7 mice). Data are represented as individual mouse data points pooled from two independent experiments. One-way ANOVA with Tukey’s multiple comparison test. (C) Serum IL-6 levels after IL-1β injection in the control and IL-1β–responsive BNST neuron-ablated group (dtA[−], n = 5 mice; dtA[+], n = 5 mice). Data are represented as individual mouse data points pooled from two independent experiments. (D) Core body temperature 6 h after IL-1β injection in the control and IL-1β–responsive BNST neuron-ablated group (dtA[−], n = 4 mice; dtA[+], n = 4 mice). (E) Serum corticosterone level after IL-1β injection in the control and IL-1β–responsive BNST neuron-ablated group (dtA[−], n = 5 mice; dtA[+], n = 5 mice). Data are represented as individual mouse data points pooled from two independent experiments. *P < 0.05.