Figure 4.
Macrophage activation profile. (A) SpatialFeaturePlot showing the expression of the myeloid cluster from an scRNA study integrated into our ST dataset. White arrows indicate myeloid hot spots. (B) Percentage of spots out of total biopsy above myeloid-probability score of 0.5 (50%) as defined from the scRNA Myeloid-1 Cluster. (C) Percent contribution of spots expressing M1 macrophage (Mφ) markers (green), M2 macrophage markers (black), or both M1 and M2 markers (orange) within the CD68+ spots. (D) Left: percentage of CD68+ that express M1 cytokines (IL-1β, TNFα, IL-12, IL-18). Right: percentage of CD68+ that express M2 cytokines (IL-10, ARG, TGFβ). (E) Multiplex IHC for CD16 (M1 macrophages), CD163 (M2 macrophages), and ACTA2 (myofibroblasts). White arrows indicate M1 macrophages (CD16); black arrows indicate M2 macrophages (CD163). P values were calculated with two-tailed t test. *P < 0.05; **P < 0.01.

Macrophage activation profile. (A) SpatialFeaturePlot showing the expression of the myeloid cluster from an scRNA study integrated into our ST dataset. White arrows indicate myeloid hot spots. (B) Percentage of spots out of total biopsy above myeloid-probability score of 0.5 (50%) as defined from the scRNA Myeloid-1 Cluster. (C) Percent contribution of spots expressing M1 macrophage (Mφ) markers (green), M2 macrophage markers (black), or both M1 and M2 markers (orange) within the CD68+ spots. (D) Left: percentage of CD68+ that express M1 cytokines (IL-1β, TNFα, IL-12, IL-18). Right: percentage of CD68+ that express M2 cytokines (IL-10, ARG, TGFβ). (E) Multiplex IHC for CD16 (M1 macrophages), CD163 (M2 macrophages), and ACTA2 (myofibroblasts). White arrows indicate M1 macrophages (CD16); black arrows indicate M2 macrophages (CD163). P values were calculated with two-tailed t test. *P < 0.05; **P < 0.01.

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