ST deconvolution and cluster validation. (A) Left: UMAP grouped by sample with defined epidermis and dermis areas. Right: UMAP grouped by cluster and biopsy. (B) Clustree to identify ideal cluster resolution. The red box indicates chosen resolution (0.8). (C) Multimodal integration analysis (MIA) showing the relative expression of structural cell types (top) and immune (bottom) clusters from an scRNA study in each of our ST cluster. (D) SpatialFeaturePlot showing the expression of the clusters annotated as VSMC-1, Fibroblasts-1, Keratinocyte-5, and Melanocyte from an scRNA study integrated into our ST samples. (E) UniCell deconvolution of a representative control and three patient lesional biopsies.