Evaluation of the L840R TLR7 variant function. Luciferase assay using HEK293T cells transfected with the pGL4.32 luciferase reporter construct and an expression vector for Renilla luciferase together with empty vector (Mock), or vectors expressing wild type (WT), LOF V795F TLR7 (negative control), GOF F507S and GOF L528I TLR7 (positive controls), or the L840R TLR7 variant stimulated or not by R848 TLR7 ligand. The results of four independent experiments are represented.