Gating strategy and representative flow cytometry plots for the NHEJ and HR measurements. (A) Debris was excluded based on forward-side scatter plots (left panel). Single transfections of mock transfected, DsRed alone, or GFP alone were used to determine the positive populations in each channel. (B) Representative histograms for the two siblings with variants in DCLRE1C and two healthy controls transfected with either NHEJ or HR reporter plasmid. (C) Bar graph of the repair efficiency (no. of GFP-positive cells/no. of DsRed-positive cells [%]) for the two siblings after HSCT and two healthy controls. The controls were assigned arbitrary numbers. Error bars indicate the standard deviation of two technical replicates.