Gating strategy and representative flow cytometry plots for the CDA. (A) Debris was excluded based on forward-side scatter plots. (B) Histogram of events versus fluorescence in the FITC channel was used to distinguish the EdU-Alexa Fluor 488–positive cells. (C) Representative histograms for the two siblings with variants in DCLRE1C and two heathy controls treated with the indicated doses of radiation or left untreated after HSCT. (D) Bar graph of the CDA index (no. of EdU-positive cells in treated sample/no. of EdU-positive cells in untreated sample [%]) for the two siblings after HSCT and two heathy controls treated with radiation at the indicated doses. The controls were assigned arbitrary numbers. Error bars indicate the standard deviation of three technical replicates for the patients and two replicates for the controls.