Figure S4.
Altered chromatin accessibility and CTCF activity in Pik3cdE1020K/+Th2 cells. Supporting data for Fig. 6. (A and B) Naïve CD4 T cells from WT and Pik3cdE1020K/+ mice underwent Th2 polarization and were examined by ATACseq. n = 3. Volcano plot showing WT-specific peaks in red and Pik3cdE1020K/+-specific peaks in blue (fold change >1.5, P < 0.05) (B) CTCF CUT&Tag tracks of Id3, Bcl2, Tbx21, and Eomes loci. (C) Quantification of CTCF protein expressed as a ratio of CTCF/Zap70. Supporting data for Fig. 5 H. n = 3 for each group, from three independent experiments. (D) Quantification of CTCF protein expressed as a ratio of CTCF/Zap70. Supporting data for Fig. 5 I. n = 3 for each group, from three independent experiments. Statistical comparisons were made using ratio paired t tests. *P < 0.05.

Altered chromatin accessibility and CTCF activity in Pik3cd E1020K/+ Th2 cells. Supporting data for Fig. 6. (A and B) Naïve CD4 T cells from WT and Pik3cdE1020K/+ mice underwent Th2 polarization and were examined by ATACseq. n = 3. Volcano plot showing WT-specific peaks in red and Pik3cdE1020K/+-specific peaks in blue (fold change >1.5, P < 0.05) (B) CTCF CUT&Tag tracks of Id3, Bcl2, Tbx21, and Eomes loci. (C) Quantification of CTCF protein expressed as a ratio of CTCF/Zap70. Supporting data for Fig. 5 H. n = 3 for each group, from three independent experiments. (D) Quantification of CTCF protein expressed as a ratio of CTCF/Zap70. Supporting data for Fig. 5 I. n = 3 for each group, from three independent experiments. Statistical comparisons were made using ratio paired t tests. *P < 0.05.

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