Figure S1. Altered responses to HDM in... | Rockefeller University Press

Figure S1.

Altered responses to HDM in Pik3cdE1020K/+mice. Supporting data for Fig. 1. (A) iBALT area (μm2) measured in H&E-stained lung sections. (B) Mucosal area (μm2) measured in H&E-stained lung sections. (A and B)n = 5–7 for each group, pooled from two independent experiments. (C) Frequencies of the indicated lung CD45+ immune cell populations (clusters 0–12) identified by scRNAseq from naïve WT, naïve Pik3cdE1020K/+, HDM-treated WT, and HDM-treated Pik3cdE1020K/+ animals from Fig. 1 G. (D) Pathway enrichment analysis using genes upregulated in HDM-treated Pik3cdE1020K/+ cells relative to HDM-treated WT counterparts for indicated cell types using scRNAseq data of lung CD45+ immune cells. Pathway enrichment was performed using ShinyGo (Ge et al., 2020). (E) Cluster-specific analysis of frequencies of cells with the indicated enrichment P value for the response to IFNγ gene set. Statistical comparison of WT and Pik3cdE1020K/+ HDM-treated groups for each cluster was performed (chi-squared test), comparing frequencies of cells with P < 0.0005. Unless otherwise indicated, statistical comparisons were made using unpaired t tests. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Altered responses to HDM in Pik3cd ^E1020K/+ mice. Supporting data for Fig. 1. (A) iBALT area (μm²) measured in H&E-stained lung sections. (B) Mucosal area (μm²) measured in H&E-stained lung sections. (A and B)n = 5–7 for each group, pooled from two independent experiments. (C) Frequencies of the indicated lung CD45⁺ immune cell populations (clusters 0–12) identified by scRNAseq from naïve WT, naïve Pik3cd^E1020K/+, HDM-treated WT, and HDM-treated Pik3cd^E1020K/+ animals from Fig. 1 G. (D) Pathway enrichment analysis using genes upregulated in HDM-treated Pik3cd^E1020K/+ cells relative to HDM-treated WT counterparts for indicated cell types using scRNAseq data of lung CD45⁺ immune cells. Pathway enrichment was performed using ShinyGo (Ge et al., 2020). (E) Cluster-specific analysis of frequencies of cells with the indicated enrichment P value for the response to IFNγ gene set. Statistical comparison of WT and Pik3cd^E1020K/+ HDM-treated groups for each cluster was performed (chi-squared test), comparing frequencies of cells with P < 0.0005. Unless otherwise indicated, statistical comparisons were made using unpaired t tests. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.