CUT&Tag identifies H3K4me1 peaks and is distinct from ATAC. To validate that the CUT&Tag assay for H3K4me1 identifies true histone marks over potential background Tn5 activity, we performed the ATACseq on paired samples from the same subject collected simultaneously as the CUT&Tag assay. Peaks were identified based on CUT&Tag reads, and reads within the peaks were quantified for both CUT&Tag and ATAC experiments. The results show greater consistency within each assay group rather than across, regardless of treatment. Reads are normalized across ATAC and CUT&Tag independently. (A) Spearman correlation of reads within the same peaks for CUT&Tag and ATAC experiments. (B) Genome browser track of reads within an identified CUT&Tag peak showing minimal reads within the same peak in an ATAC experiment. (C) Genome browser track of reads within an identified CUT&Tag peak showing higher reads within the same peak in an ATAC experiment. (D) Genome browser track of reads within an identified CUT&Tag peak showing similar reads within a peak between ATAC and CUT&Tag experiments.