Figure S6.
αII-spectrin–actomyosin networks regulate the EGFR–TRPV3–TGM pathway. (a) Dorsal skin sections from shCtr and shSptan1 0595-transduced E17.5 embryos immunolabeled for EGFR. Upper Insets show the transduced cells (H2B−GFP+). (b) Dorsal skin sections from shCtr; TRPV3-GFP–transduced E17.5 embryos immunolabeled for pEGFR. (c) Dorsal skin sections from shSptan1 9753; TRPV3-GFP–transduced E17.5 embryos. Insets show the magnification of the boxed area from each epidermal layer. (d) Primary mouse keratinocytes cultured in high-calcium (1.5 mM) medium treated with DMSO, latrunculin, or Y27632 and immunolabelled for E-cadherin and TRPV3. Overviews corresponding to Fig. 6 m. Boxes indicate the location of the magnified area. (e) Quantification of E-cadherin intensity. Mean ± SD from ∼150 mature junctions from n = 3 experiment per condition with Kolmogorov–Smirnov. Bars: mean normalized intensity; dots: mature junctions. Nuclei were stained with DAPI.

αII-spectrin–actomyosin networks regulate the EGFR–TRPV3–TGM pathway. (a) Dorsal skin sections from shCtr and shSptan1 0595-transduced E17.5 embryos immunolabeled for EGFR. Upper Insets show the transduced cells (H2B−GFP+). (b) Dorsal skin sections from shCtr; TRPV3-GFP–transduced E17.5 embryos immunolabeled for pEGFR. (c) Dorsal skin sections from shSptan1 9753; TRPV3-GFP–transduced E17.5 embryos. Insets show the magnification of the boxed area from each epidermal layer. (d) Primary mouse keratinocytes cultured in high-calcium (1.5 mM) medium treated with DMSO, latrunculin, or Y27632 and immunolabelled for E-cadherin and TRPV3. Overviews corresponding to Fig. 6 m. Boxes indicate the location of the magnified area. (e) Quantification of E-cadherin intensity. Mean ± SD from ∼150 mature junctions from n = 3 experiment per condition with Kolmogorov–Smirnov. Bars: mean normalized intensity; dots: mature junctions. Nuclei were stained with DAPI.

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