Autophagy flux of Keap1, p62, and LC3. (A) Western blot analysis of whole-cell lysates (WCL) from WT, SHKBP1 KO, and FLAG-SHKBP1–OE HeLa cells treated with As(III) (10 µM) and BafA1 (100 nM) for 0 or 6 h. (B–D) Quantification of Keap1, p62, and LC3-II to demonstrate the turnover of endogenous p62, Keap1, and LC3-II, with band intensities normalized to actin levels (n = 3). Exact P values indicated from two-way ANOVA with Tukey’s post hoc test. Source data are available for this figure: SourceData FS4.