Intracellular colocalization of p62 and LC3. (A) Confocal microscopy analysis of WT, SHKBP1 KO, and OE HeLa cells cotransfected with GFP-p62 (green) and mScarlet-i-LC3 (magenta) with DMSO or BafA1 treatment. Scale bars: 10 μm. (B) Quantification of colocalization between Keap1 and p62. n = 6 images from three independently plated samples. Statistical analysis from two-way ANOVA with Tukey’s post hoc test.