Zbtb32 regulates the epigenetic landscape of CD8 ⁺ TILs. (A) Distribution of differential chromatin accessible regions in WT and Zbtb32^−/− OT-I TILs across the genome. (B) Enrichment of known TF-binding motifs in chromatin accessible regions between WT and Zbtb32^−/− OT-I TILs. (C) Volcanic plot showing differential chromatin accessible regions between WT and Zbtb32^−/− OT-I TILs. (D) Lists of top 20 GO enrichment pathways for differential chromatin accessible genes in WT and Zbtb32^−/− OT-I TILs. (E) Chromatin accessible peaks at several classic gene loci in WT and Zbtb32^−/− OT-I TILs aligned with assay for ATAC-seq tracks of T_tex and T_pex TILs from B16-OVA tumor. Binding peaks on Prdm1, Batf, Id2, Havcr2, Il2rb, Gzmb, and Gzmk loci are shown (GEO accession: GSE123236). (F) PSEA results for a comparison of the enrichment of epigenetic signature peaks in T_pex and T_tex CD8⁺ T cell subsets in WT and Zbtb32^−/− OT-I TILs (GEO accession: GSE123236). PSEA, peak set enrichment analysis.