Zbtb32 exerts a long-term influence on CD8 ⁺ T cells in tumor, but not in other immune organs. (A) Representative plots and quantifications of WT and Zbtb32^−/− OT-I cells in TILs, DLN, and SPL (n = 5 in each group). (B) Representative plots (left panel) and quantifications (right panel) of TCF1 and Tim-3 in WT and Zbtb32^−/− OT-I cells in TILs, DLN, and SPL (n = 5 in each group). (C) Schematic diagram of the co-transfer of 1.5 × 10⁵ WT and 1.5 × 10⁵Zbtb32^−/− naïve OT-I cells before 1 × 10⁶ B16-OVA inoculation and collection at early and late time points (n = 5 in each group). (D) Representative plots and quantifications of WT and Zbtb32^−/− OT-I cells in TILs at different time points (n = 5 in each group). (E) Representative plots (left panel) and quantifications (right panel) of TCF1 and Tim-3 in WT and Zbtb32^−/− OT-I cells in TILs at different time points (n = 5 in each group). Data in all graphs are shown as means ± SEM; ns, not significant; *P < 0.05, **P < 0.01 and ****P < 0.0001 by paired two-tailed Student’s t test. Data shown are a representative of two independent experiments.