Zbtb32 expression is selectively upregulated in T _tex tumor-infiltrating CD8 ⁺ T cells. (A) Fold change in the expression level of different TFs in T_pex and T_tex OT-I TILs in E.G7. (B) Volcanic plot showing the differential expression of TFs between T_pex and T_tex OT-I TILs in E.G7. (C) FeaturePlots showing Cd8a and Zbtb32 expression profiles in B16 TILs. (D) The expression levels of Zbtb32 and Cd8a in different clusters of B16 TILs. (E) The tSNE plot of scRNA-seq dataset, indicating global transcriptomic similarities of CD8⁺ TILs in B16 TME. (F) FeaturePlots showing expression profiles of Zbtb32 and other classic genes in B16 CD8⁺ TILs. (G)Zbtb32 and other classic genes expression levels in different clusters of B16 CD8⁺ TILs. (H) RT-qPCR analysis of Zbtb32 expression in Ly108⁺ and Tim-3⁺ CD8⁺ TILs (n = 3 for each group). GEO accessions: (A and B) GSE182035, (C–G) GSE122675. Unpaired two-tailed Student’s t test (H), ***P < 0.001. Data shown are a representative of at least two independent experiments.