Unilateral furrowing requires the linker region and the C-terminus of ANI-1. (A) Eccentricity of ring closure was calculated as shown in schematics (left) and plotted for different percentages of ring constriction for the indicated conditions (right). (B) Mean peak eccentricity of the ring during constriction for the indicated control, and GFP::ANI-1 or FLAG::ANI-1 expressing embryos with and without ani-1(RNAi). (C) Leading and lagging edge progression over time for control and ani-1(RNAi) treated embryos. Time zero represents the onset of furrow ingression. The time difference when the leading and lagging edge reach 10 % edge progression represents the Lag time. (D) Mean Lag time between the leading and lagging edge for indicated conditions. For all, error bars are SEM, P values were calculated using two-tailed Student’s t test or Mann–Whitney U test and are n.s. P > 0.05, *P < 0.05, **P < 0.01 and ***P < 0.001.