The linker region is not targeted to the leading edge but if connected with the C-terminus they localize asymmetrically in the ring. (A) End-on reconstruction of indicated GFP::ANI-1 variants at the cleavage plane for highlighted % of ring constriction treated with or without ani-1(RNAi). Scale bars is 5 µm. Since the localization of indicated GFP::ANI-1 variants to the ring is very low their intensity scaling was increased. For comparison the panels on the right have the same intensity scaling as for GFP::ANI-1^WT at 50% constriction. White stars indicate the position of the leading edge. (B and C) Total GFP::ANI-1 intensity in the ring (B) and ratio of the leading and lagging edges of GFP::ANI-1 (C) for different ANI-1 variants were calculated at 50% ring constriction. P values were calculated using two-tailed Student’s t test or Mann–Whitney U test and are n.s. P > 0.05, *P < 0.05, **P < 0.01 and ***P < 0.001, n = number of embryos, error bars are SEM.