ANI-1 limits the accumulation of NMY-2::mKate and Formin ^CYK-1 ::GFP but not IT-RHO-1 in the ring. (A) End-on reconstruction of NMY-2::mKate at the cleavage plane for indicated % of ring constriction with and without ani-1(RNAi). (B) Total ring intensity was measured at 50% constriction after the background (BG) intensity was subtracted (top). Total ring intensity of NMY-2::mKate, Formin^CYK-1::GFP, and IT-RHO-1 expressing embryos treated with or without ani-1(RNAi) (bottom). P values were calculated using two-tailed Student’s t test and are n.s. P > 0.05 and ***P < 0.001, n = number of embryos, and error bars are SEM. (C) End-on reconstruction of Formin^CYK-1::GFP at the cleavage plane for indicated % of ring constriction of embryos with or without ani-1(RNAi). (D) End-on reconstruction of IT-RHO-1 at the cleavage plane for indicated % of ring constriction of embryos with or without ani-1(RNAi). All scale bars are 5 µm.