Latrunculin A–induced IT-RHO-1 structures depend on ANI-1. (A) The length (cyan) and width (magenta) of IT-RHO-1 structures were determined at the cell equator after the application of different filters on the original IT-RHO-1 images. Images are reproduced from Fig. 3 A. IT-RHO-1 structures with a length/width ratio of ≥4 were classified as linear and structures with a length/width ratio of <4 as non-linear (Lebedev et al., 2023). (B) Maximum intensity projections of 10 cortical z-planes of permeabilized one-cell control and ani-1(RNAi) embryos treated with DMSO or Latrunculin A (LatA) as indicated. A zoom-in of the equatorial region for the indicated time points is shown on the right. Scale bars are 5 µm. (C) Mean number of linear and non-linear IT-RHO-1 structures at the equatorial region after LatA treatment for control and ani-1(RNAi) embryos for indicated time points after NEBD. (D) Mean IT-RHO-1 intensity from the anterior to the posterior poles for LatA-treated embryos with and without ani-1(RNAi) treatment at indicated time points after NEBD. (E) Mean normalized fluorescence intensity of IT-RHO-1 at the cell equator for LatA-treated embryos with and without ani-1(RNAi) treatment at indicated time points after NEBD. All error bars are SEM and dots represent data points of individual embryos. P values were calculated using two-tailed Student’s t test and are n.s. P > 0.05.