Loss of uPA or of its cell surface localization alters DC entry into lymphatics. Quantitative whole-mount analysis of endogenous DC positioning in the steady-state ear skin of WT, uPA^mut, uPA^−/−, and CCR7^−/− mice. (A) Top row: Representative confocal images from the four genotypes. DCs are identified as CD45⁺CD11c⁺ cells (yellow). Bottom row: Confocal images with orthogonal views provided for one selected DC in the upper row image. Scale bars: 50 μm. The number in each image indicates the DC tissue position with respect to the LV, as defined in B. (B) Schematic depiction of the three different types of DC tissue positionings: (1) interstitial space, (2) adherent to the outer surface of the LV, and (3) within the LV lumen. (C) Quantification of the percentage DCs colocalized with lymphatics (i.e., percentage of (2+3)/(1+2+3), as defined in B). (D) Quantification of the percentage DCs localized inside the LV lumen (i.e., percentage of (3)/(1+2+3), as defined in B). Data points from the same experiment (involving one mouse per genotype) are connected by a line. n = 4–10 mice per condition. Paired Student’s t test.