land-ExM coupled with immunostaining for cytoskeleton visualization. (A–C) land-ExM total lipid (magenta) and protein (green) images of breast cancer cells stained with phalloidin-FITC and anti-FITC antibody (yellow). Scale bar: 5 µm in pre-expansion unit. Linear expansion factor: 3.8. (D–G) Zoom in images of A–C showing details of F-actin. Scale bar: 500 nm in pre-expansion unit. (H) Intensity profile along the gray line in image (D). All images were taken with an Airyscan microscope.