land-ExM using proteinase K digestion. (A) Workflow of land-ExM using proteinase K digestion to homogenize cells instead of heat denaturation. (B) land-ExM protein image of U2OS cells with proteinase K digestion (proK). Scale bar: 10 µm in pre-expansion unit. Linear expansion factor: 4.0. (C) land-ExM protein image of U2OS cells with heat denaturation (heat). Scale bar: 10 µm in pre-expansion unit. Linear expansion factor: 4.0. (D) land-ExM lipid image of U2OS cell with proteinase K digestion (proK). Scale bar: 10 µm in pre-expansion unit. Linear expansion factor: 4.0. (E) land-ExM lipid image of U2OS cells with heat denaturation (heat). Scale bar: 10 µm in pre-expansion unit. Linear expansion factor: 4.0. All images were taken with an Airyscan microscope. Image in C is also shown in Fig. 1 D.