Figure 7.
FAs redistribute from protrusions in mesenchymal clusters to internal regions of the cluster in epithelial-like clusters during chemotaxis toward SDF1. (A) Immunofluorescence staining of mesenchymal and epithelial-like NC clusters in the absence of external chemotactic cues, labeled for pPAX and nuclei. The region shown corresponds to edge protrusions. (B and C) Quantification of FA area and number at edge protrusions in NC clusters shown in A (n = 10 clusters per condition). (D) Immunofluorescence staining of mesenchymal and epithelial-like NC clusters in the absence of external chemotactic cues, labeled for pPAX and nuclei. The region shown is the inner cluster region. (E and F) Quantification of FA area and number in internal regions of the cluster in NC clusters shown in D (n = 10 clusters per condition). (G) Immunofluorescence staining of mesenchymal and epithelial-like NC clusters during chemotaxis toward SDF1, labeled for pPAX and nuclei. The region shown corresponds to edge protrusions. (H and I) Quantification of FA area and number at edge protrusions in NC clusters shown in G (n = 10 clusters per condition). (J) Immunofluorescence staining of mesenchymal and epithelial-like NC clusters during chemotaxis toward SDF1, labeled for pPAX and nuclei. The region shown corresponds to the cluster interior. (K and L) Quantification of FA area and number in the internal regions in NC clusters shown in J (n = 10 clusters per condition). Scale bars: 30 µm. Each dot represents one cluster. Data are from at least three independent experiments. Error bars show mean ± SEM. Error bars show mean ± SEM. Statistical analysis was performed using unpaired two-tailed Student’s t test. ****P ≤ 0.0001; ***P < 0.001; **P ≤ 0.01.

FAs redistribute from protrusions in mesenchymal clusters to internal regions of the cluster in epithelial-like clusters during chemotaxis toward SDF1. (A) Immunofluorescence staining of mesenchymal and epithelial-like NC clusters in the absence of external chemotactic cues, labeled for pPAX and nuclei. The region shown corresponds to edge protrusions. (B and C) Quantification of FA area and number at edge protrusions in NC clusters shown in A (n = 10 clusters per condition). (D) Immunofluorescence staining of mesenchymal and epithelial-like NC clusters in the absence of external chemotactic cues, labeled for pPAX and nuclei. The region shown is the inner cluster region. (E and F) Quantification of FA area and number in internal regions of the cluster in NC clusters shown in D (n = 10 clusters per condition). (G) Immunofluorescence staining of mesenchymal and epithelial-like NC clusters during chemotaxis toward SDF1, labeled for pPAX and nuclei. The region shown corresponds to edge protrusions. (H and I) Quantification of FA area and number at edge protrusions in NC clusters shown in G (n = 10 clusters per condition). (J) Immunofluorescence staining of mesenchymal and epithelial-like NC clusters during chemotaxis toward SDF1, labeled for pPAX and nuclei. The region shown corresponds to the cluster interior. (K and L) Quantification of FA area and number in the internal regions in NC clusters shown in J (n = 10 clusters per condition). Scale bars: 30 µm. Each dot represents one cluster. Data are from at least three independent experiments. Error bars show mean ± SEM. Error bars show mean ± SEM. Statistical analysis was performed using unpaired two-tailed Student’s t test. ****P ≤ 0.0001; ***P < 0.001; **P ≤ 0.01.

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