E-cadherin alters the directional organization of cell movements within NC clusters during chemotaxis toward SDF1. (A and B) Time color-coded images of nuclear trajectories in epithelial-like (A) and mesenchymal (B) NC clusters undergoing chemotaxis toward SDF1. (C and D) Tracking of nuclear positions from A and B. Scale bar: 50 µm. (E and F) Rose plots showing the angular distribution of nuclear movements from C and D, aligned to the main axis of cluster migration. (G and H) Quantification of nuclear speed (G) and persistence (H) based on nuclear tracks. Data are representative of at least three independent experiments. Error bars indicate mean ± SEM. Statistical analysis was performed using unpaired two-tailed Student’s t test; **P ≤ 0.01; n.s., not significant.