Characterization of the MDA-MB-231 Vcl KO cell line. (A) Quantification of the percentage of dead cells from 30 fields of view per condition using a LIVE/DEAD kit (N = 3). (B) Representative images of cell lines indicated treated with a LIVE/DEAD kit to assess cell viability in 2D. The right panel represents the positive control condition. Scale bar, 200 µm. (C and D) Percentage of EdU incorporation in parental MDA-MB-231 and Vcl KO cells and (D) representative images of EdU incorporation and DAPI to assess cell proliferation (N = 3). Scale bar, 100 µm. Graphs denote the mean ± SEM. ns = not significant.