Figure S1.
Representative western blots. (A) Representative western blot confirming Vcl knockdown in MDA-MB-231 cells expressing PercevalHR and pHRed probes, including a scrambled control (siCtrl) and siVcl. Relevant samples are outlined with a red box, and uncropped membrane is included to show the ladder. (B and C) Representative western blot and (C) quantification from three independent western blots confirming Vcl KO and rescue in MDA-MB-231 cells (N = 3). The Pre-Sort population refers to the Vcl KO cells transduced with Vcl-Venus before positively expressing Venus cells were sorted using FACS. Bar graph denotes the mean ± SEM. ns = not significant. (D) Representative western blot to confirm Vcl KO in NIH/3T3 cells. (E) Representative western blot to confirm Vcl KO in MCF10A cells. siVcl, siRNA targeting vinculin; FACS, fluorescence-activated cell sorting. Source data are available for this figure: SourceData FS1.

Representative western blots. (A) Representative western blot confirming Vcl knockdown in MDA-MB-231 cells expressing PercevalHR and pHRed probes, including a scrambled control (siCtrl) and siVcl. Relevant samples are outlined with a red box, and uncropped membrane is included to show the ladder. (B and C) Representative western blot and (C) quantification from three independent western blots confirming Vcl KO and rescue in MDA-MB-231 cells (N = 3). The Pre-Sort population refers to the Vcl KO cells transduced with Vcl-Venus before positively expressing Venus cells were sorted using FACS. Bar graph denotes the mean ± SEM. ns = not significant. (D) Representative western blot to confirm Vcl KO in NIH/3T3 cells. (E) Representative western blot to confirm Vcl KO in MCF10A cells. siVcl, siRNA targeting vinculin; FACS, fluorescence-activated cell sorting. Source data are available for this figure: SourceData FS1.

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