sis2Δ mutant behaves like a hypomorphic TRK1 allele. (A) Internal potassium concentration. Data are from two independent experiments with three replicates per experiment per strain. (B) Plasma membrane potential, as measured by the ANEPPS ratio (see Materials and methods for details). Ratios are normalized to the WT value. Data are from five experiments with 3–4 technical replicates per genotype per experiment. (C) MMP ratio as measured by the genomic reporter (Fig. 1 A) and normalized to WT. Data are from two experiments with three technical replicates for each genotype. (D) Kaplan–Meier lifespan estimates. Data are from four (WT, sis2Δ, trk1Δ) or two (sis2Δtrk1Δ) experiments. n = 6,333 for WT, n = 5,871 for sis2Δ, n = 7,359 for trk1Δ, n = 4,632 for sis2Δtrk1Δ. (E) log(HR) comparisons of data from D. In all panels, error bars show 95% confidence intervals.