Lifespan and MMP phenotypes of sat4Δ and hal5Δ mutants are additive with sis2Δ. (A) Kaplan–Meier lifespan estimates. n = 6,500 for WT, n = 4,806 for sis2Δ, n = 6,874 for sat4Δ, n = 5,542 for hal5Δ, n = 4,563 for sis2Δsat4Δ, n = 4,874 for sis2Δhal5Δ, n = 2,348 for sat4Δhal5Δ, n = 535 for sis2Δsat4Δhal5Δ. Data are from three experiments, but technical issues reduced the representation of the sat4Δhal5Δ and sis2Δsat4Δhal5Δ strains to one unique experiment each. (B) log(HR) from a CPH model of A. See legend of Fig. 2 for details. (C) MMP for strains in A, as measured using TMRM. Values are normalized to the mean WT value. Data are from two experiments, 20–28 replicates per genotype and 10,000 cells analyzed per replicate. (D) Culture doubling times, as measured after 24 h of exponential growth before lifespans (A) and MMP (C), were measured. Data are from three experiments; 7–9 replicates per genotype. In all panels, error bars show 95% confidence intervals.